Functional characterisation of twelve terpene synthases from actinobacteria

• Anuj K. Chhalodia,
• Houchao Xu,
• Georges B. Tabekoueng,
• Binbin Gu,
• Kizerbo A. Taizoumbe,
• Lukas Lauterbach and
• Jeroen S. Dickschat

Beilstein J. Org. Chem. 2023, 19, 1386–1398, doi:10.3762/bjoc.19.100

• Chitinophaga pinensis [29][30], but no α-cadinene synthase has been reported to date. The enzyme newly described here was designated as Streptomyces jumonjiensis (+)-α-Cadinene Synthase (SjaCS). A few more enzymes with a pairwise identity of 83% are observed in other actinomycetes that likely also function as

18-Hydroxydolabella-3,7-diene synthase – a diterpene synthase from Chitinophaga pinensis

• Jeroen S. Dickschat,
• Jan Rinkel,
• Patrick Rabe,
• Arman Beyraghdar Kashkooli and
• Harro J. Bouwmeester

Beilstein J. Org. Chem. 2017, 13, 1770–1780, doi:10.3762/bjoc.13.171

• Wageningen, The Netherlands, Swammerdam Institute for Life Sciences, University of Amsterdam, Sciencepark 904, 1098 XH Amsterdam, The Netherlands 10.3762/bjoc.13.171 Abstract The product obtained in vitro from a diterpene synthase encoded in the genome of the bacterium Chitinophaga pinensis, an enzyme

• , revealing that the expression of one and the same terpene synthase in different heterologous hosts may yield different terpene products. Keywords: biosynthesis; Chitinophaga pinensis; Nicotiana benthamiana; structure elucidation; terpenes; Introduction Terpene synthases convert a handful of simple linear

• production of volatile terpenes by this bacterium that can be detected in headspace extracts [17][18]. In one of these previous reports [17] we have described a terpene synthase from Chitinophaga pinensis DSM 2588 (accession number WP_012789469) as a sesquiterpene synthase for germacrene A (1), which was

Mechanistic investigations on six bacterial terpene cyclases

• Patrick Rabe,
• Thomas Schmitz and
• Jeroen S. Dickschat

Beilstein J. Org. Chem. 2016, 12, 1839–1850, doi:10.3762/bjoc.12.173

• relative (94% identical sites). Another terpene synthase from Chitinophaga pinensis DSM 2588 (accession number WP_012792334) converted FPP into a terpene hydrocarbon 5, identified as γ-cadinene by GC–MS [32], besides traces of α- and δ-cadinene (Figure S1, Supporting Information File 1), while no reaction

• synthases from Streptomyces viridochromogenes ((−)-α-amorphene (1) and (−)-7-epi-α-eudesmol synthase (4)), the (+)-T-muurolol synthase (2) from Roseiflexus castenholzii, the (+)-4-epi-cubebol synthase (3) from Streptosporangium roseum, the (−)-γ-cadinene synthase (5) from Chitinophaga pinensis and the